. Author manuscript; available in PMC: 2019 Nov 28.

Published in final edited form as: J Am Chem Soc. 2018 Nov 12;140(47):16115–16123. doi: 10.1021/jacs.8b08416

Table 4.

In vivo replication data

Sequence Contexts 1–4 1: 5’-CTCGAGTACAACTTTAACTCACACAATGTCA-X-TGTCACGGCAGACAAACAAAAGAATGGAATC 2: 5’-CTCGAGTACAACTTTAACTCACACAATGTCC-X-CGTCACGGCAGACAAACAAAAGAATGGAATC 3: 5’-CTCGAGTACAACTTTAACTCACACAATGTCC-X-GGTCACGGCAGACAAACAAAAGAATGGAATC 4: 5’-CTCGAGTACAACTTTAACTCACACAATGTCC-X-AGTCACGGCAGACAAACAAAAGAATGGAATC

SSO	Sequence Context	dXTP	retention (%)

			[dXTP]
			150 μM	25 μM	10 μM	5 μM
YZ3	1	dNaMTP	98 ± 4	82 ± 15	29 ± 11	17 ± 21
		dPTMOTP	98 ± 5	99 ± 0.5	92 ± 6	77 ± 22
		dMTMOTP	99.0 ± 1.2	96 ± 5	80 ± 11	59 ± 24
	2	dNaMTP	44 ± 15	7 ± 3	–^a	–^a
		dPTMOTP	97 ± 10	55 ± 14	–^a	–^a
		dMTMOTP	62 ± 22	18.7 ± 0.6	–^a	–^a
	3	dNaMTP	6 ± 3	4.8 ± 1.2	–^a	–^a
		dPTMOTP	7 ± 4	2 ± 4	–^a	–^a
		dMTMOTP	7.9 ± 0.7	5 ± 4	–^a	–^a
ML1	3	dNaMTP	28 ± 14	9 ± 2	–^a	–^a
		dPTMOTP	60 ± 15	19 ± 6	–^a	–^a
		dMTMOTP	25 ± 8	10 ± 4	–^a	–^a
	4	dNaMTP	26 ± 18	11.0 ± 1.4	–^a	–^a
		dPTMOTP	41 ± 19	21±10	–^a	–^a
		dMTMOTP	22 ± 18	12 ± 5	–^a	–^as

Not determined