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. Author manuscript; available in PMC: 2020 Feb 12.
Published in final edited form as: Biochemistry. 2019 Jan 7;58(6):561–574. doi: 10.1021/acs.biochem.8b01066

Figure 3.

Figure 3.

Representative autoradiographs of denaturing gels of nucleotide excision experiments in HeLa cell extracts. (A) The substrates were either free 147-mer 601cP DNA sequences containing single 5’,8-cyclopurine lesions, or nucleosomes assembled with octamers derived from recombinant (Rec) or native, post-translationally modified histones extracted from HeLa cells. (B) Analogous NER experiments with cis- and trans-BPDE-dG adducts positioned at the same In and Out superhelical locations. Three separate gels are depicted in panels A and B.