Table 1.

Sequence, Concentration and Part Number of Primer and Probes

	Sequence	Concentration
RASSF1A primers
Forward primer	5’-AGCCTGAGCTATTGAGCTG-3’	40,000 pmol
Reversed primer	5’-CGGCGGATCGGCAAA-3’	40,000 pmol
RASSF1A probe	5’FAMTM-CCAACGCGCTGCGCAT(MGBTM)-3’	5,000 pmol
β -actin primers
Forward primer	5’-GCGCCGTTCCGAAAGTT-3’	40,000 pmol
Reversed primer	5’-CGGCGGATCGGCAAA-3’	40,000 pmol
β-actin probe	5’VICTM-ACCG CCGAGACCGCGTC(MGBTM)-3’	5,000 pmol