Figure 4.

Effect of DBP exposure on cell cycle progression gene expression in cultured antral follicles. Early antral follicles (n = 6–12 follicles per treatment) were obtained from the ovaries of untreated CD-1 mice and cultured as described in Materials and Methods section. At the end of each culture, RNA was extracted and processed for qPCR analysis of Ccnd2 (A) and Ccne1 (B). Data were normalized to housekeeping genes and are presented as mean relative expression ± SEM with asterisks (*) indicating significantly different from control.