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. 2015 Oct 9;212(9):1509–1520. doi: 10.1093/infdis/jiv221

Figure 3.

Figure 3.

Expression of Ifit1 protects against tumor necrosis factor α (TNF-α)–mediated apoptosis in vitro. A and B, Mice were treated with 0.01 µg of lipopolysaccharide (LPS) alone or together with 20 mg D-(+)-galactosamine (GalN). Livers were isolated at indicated times after injection. A, Real-time quantitative polymerase chain reaction analysis of Ifit1 in mouse livers (n = 3). Expression levels are normalized to levels in untreated samples. B, Immunoblotting for Ifit1 in mice liver. Curves represent quantification of the immunoblotting results by ImageJ software, and expression levels are normalized to that in lane 1 (n = 3). Data are presented as mean values ± SD. ***P < .001, by the 2-tailed t test, for comparisons to LPS/GalN groups. C and D, BNL CL.2 cells expressing Ifit1 or empty vector were treated with 0.01 µg/mL TNF-α and 1 µg/mL actinomycin D (ActD) simultaneously or separately for 7 hours. C, Immunoblotting analysis of caspase-3 activation. D, Flow cytometry analysis of apoptosis. Comparisons were made using the 2-tailed t test. Abbreviation: mRNA, messenger RNA.