Figure 4.

Gene transfer of Ifit1 protects mice against lipopolysaccharide (LPS)/D-(+)-galactosamine (GalN)–induced lethal hepatitis. Mice were injected intravenously with recombinant adeno-associated virus serotype 8 (rAAV8) encoding Ifit1-eGFP or eGFP or with saline and challenged 4 days later with 0.01 µg of LPS plus 20 mg of GalN. Livers and serum were harvested 7 hours after LPS/GalN challenge. A, Survival analysis of the mice injected with indicated rAAV8. B, Histological examination of hematoxylin-eosin–stained liver sections. C, Serum alanine aminotransferase (ALT) levels (n = 3). Data are presented as mean values ± SD. Comparisons were made using the 2-tailed t test. D, Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining of liver sections for apoptosis detection. All scale bars represent 100 µm. **P < .01. Abbreviations: NS, not significant; PBS, phosphate-buffered saline.