Figure 6.

Ifit1 inhibits tumor necrosis factor α (TNF-α)–induced apoptosis by binding to Axin. A–C, BNL CL.2 cells expressing Ifit1 were or were not treated with TNF-α and actinomycin D (ActD) for 5 hours. A, Coimmunoprecipitation with an anti-Flag antibody or control immunoglobulin G (IgG), followed by immunoblotting. B, Coimmunoprecipitation with an anti-Axin antibody or control IgG, followed by immunoblotting. C, Immunofluorescence staining for detecting the localization of Ifit1, MDFI, and Axin. D, BNL CL.2 cells expressing Ifit1-δN or Ifit1-δC were cotreated with TNF-α and ActD for 5 hours. Cell lysates were probed by immunoblotting for Ifit1, JNK, p38, and β-actin. Scale bar represents 30 µm. Abbreviations: JNK, c-Jun N-terminal kinase; DMSO, dimethyl sulfoxide.