Fig 2. Interaction between human CD96 and nectin-1 V-domain by ELISA.

Plates were coated with Nectin-1(143t)-MBP at 10 μg/ml, or mock treated (milk protein control). Increasing concentrations of hCD96t were added and bound hCD96t was detected using an anti-tetraHis tag Mab (Qiagen), followed by secondary antibody and substrate. Absorbance was read at 405 nm. Average values ± one standard deviation of four independent experiments are shown.