Table 1. Affinity and kinetics constants of CD96(517t) ectodomain to nectin-1(346t) ectodomain and nectin-1(143t) V-domain determined by SPR.

	Nectin1(346t)		Nectin-1(143t)
	CD96(517t)a	gD(306t)b	CD96(517t)a	gD(306t)b
KD [10−6 M]	2.43 ± 0.24	3.2	3.22 ± 0.59	1.2
kon [103 s-1M-1]	3.9 ± 1.48	2.2	3.2 ± 1.26	9
koff [10−2 s-1]	0.94 ± 0.3	0.7	1.2 ± 0.14	1.1

^a means ± one standard deviation of values from 3 independent experiments.

^b values reported by Krummenacher et al, 1999.