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. 2019 Jan 22;18:1533033818823029. doi: 10.1177/1533033818823029

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© The Author(s) 2019

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — lncINS-IGF2 knockdown results in downregulation of IGF2 in NSCLC cells. A549 and H1299 cells were transfected with control siRNA or lncINS-IGF2 siRNA for 48 hours. The cells were subsequently harvested to assess knockdown effects (A and B). A549 and H1299 cells were transfected with NC and siRNA of lncINS-IGF2. IGF2 mRNA levels were measured by qRT-PCR assay (C and D). *P < .05 versus NC. Data are expressed as the mean (SD) and each experiment was performed in triplicate. IGF2, insulin-like growth factor 2; NSCLC, non-small-cell lung cancer; siRNA, small interfering RNA; qRT-PCR, real-time quantitative polymerase chain reaction; NC, normal control; SD, standard deviation.