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. Author manuscript; available in PMC: 2019 Feb 13.
Published in final edited form as: Pharmacol Res. 2017 May;119:479–489. doi: 10.1016/j.phrs.2016.11.036

Fig. 4.

Fig. 4.

AFM force measurements with EGF-functionalized AFM tip on living KYSE-150 cells. (A) AFM tip functionalization procedure and schematic representation of the single-molecule force measurement between EGF-functionalized AFM tips and EGFR in KYSE-150 cells. (B, C) Representative force curves obtained with EGF-modified AFM tips in KYSE-150 cells, and (D) after the system was blocked with free anti-EGFR antibody. (E) The binding probability of EGF-functionalized tips in KYSE-150 cells before and after blocking, data was expressed as mean ± S.E.M. from four independent experiments, and in each experiments, more than 1000 force curves were measured on living cells, **p < 0.01.