Table 1.

Effects of oridonin on the dissociation kinetics for EGF-EGFR complex. x_β, k_off (0) and ΔG₀ are the energy barrier width, the dissociation off-rate constant and the activation energy for the EGF-EGFR complex on the surface of KYSE-150 cells, respectively.

KYSE-150 cells	xβ (nm)	koff(0) (s−1)	∆G0 (kBT)
Control	0.172 ± 0.003	0.034 ± 0.002	24.043 ± 0.114
10 μM Oridonin	0.170 ± 0.009	0.038 ± 0.002	23.987 ± 0.058
30 μM Oridonin	0.164 ± 0.013	0.049 ± 0.005**	23.743 ± 0.105*
50 μM Oridonin	0.167 ± 0.016	0.054 ± 0.003***	23.650 ± 0.061**
NAC + 50 μM Oridonin	0.169 ± 0.006	0.041 ± 0.002*##	23.977 ± 0.046##
NAC	0.174 ± 0.002	0.035 ± 0.003	24.080 ± 0.090

All groups are compared with control group, n = 3,

^*p < 0.05

^**p < 0.01

^***p < 0.001, and 2.5 μM MNAC + 50 μM oridonin treated group is also compared with 50 μM oridonin treated group

^#p < 0.05

^##p < 0.01.