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. 2019 Jan 28;8:e41028. doi: 10.7554/eLife.41028

Figure 8. DIP-α is required for the loss of MNISN-1s innervation of m4 when overexpressing Dpr10 postsynaptically.

(a) Loss of MNISN-1s innervation of m4 due to overexpression of UAS-dpr10-V5 (referred to in the figure as UAS-dpr10) in muscles with the Mef2-GAL4 driver. Dpr10 is localized specifically to the postsynaptic membrane (green) and co-localizes with Dlg, a postsynaptic membrane marker (red). Anti-HRP (blue) labels all neuronal membrane. (a1) and (a2) show the individual Dpr10 and Dlg channels, respectively. Note that only 1b terminals are present. Also, the Dpr10 protein is labeled with anti-V5. (b) Muscle overexpression of a Dpr10 variant (UAS-dpr10Y103A) that is incapable of binding DIP-α does not affect m4 innervation. Both 1b and 1 s (arrow) terminals are present on m4. The 1b and 1 s terminals are easily distinguished by size and staining intensity of Dlg (b2) (see Materials and methods). (c) Quantification of 1 s innervation of m4. Overexpression of wild type UAS-dpr10 transgene results in 25% of m4s innervated by MNISN-1s compared to 89% innervation when overexpressing UAS-dpr10Y103A which is unable to bind DIP-α. n: See Figure 8—source data 1. ***p<0.0001. Calibration bar, 10 μm.

Figure 8—source data 1. Source data for Figure 8c.
elife-41028-fig8-data1.docx (13.1KB, docx)
DOI: 10.7554/eLife.41028.025
Figure 8—source data 2. Source data for Figure 8—figure supplement 1.
elife-41028-fig8-data2.docx (12.5KB, docx)
DOI: 10.7554/eLife.41028.026

Figure 8.

Figure 8—figure supplement 1. Weak and strong GAL4 expression of the UAS-dpr10 transgene.

Figure 8—figure supplement 1.

Low level expression of UAS-dpr10 with BG487-GAL4 shows approximately wildtype levels of m4 innervation; however high level expression of UAS-dpr10 with 24B-GAL4 shows a similar phenotype to another strong muscle driver, Mef2-GAL4. See also Figure 8. n: See Figure 8—source data 2.