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. 2019 Feb 13;5(2):eaav2554. doi: 10.1126/sciadv.aav2554

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Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.

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Fig. 1 — (A) To identify endogenous receptors recognized by IAV, we generated a shotgun glycan microarray comprising the N-glycans from the human lung (7, 42, 43). Lungs (provided by LifeLink) were processed following the oxidative release of natural glycans (ORNG) method (17) and labeled with the bifunctional linker, 2-amino-N-(2-aminoethyl)-benzamide (AEAB) (40). The labeled N-glycans were printed on glass slides to generate the HL-SGM. RFU, relative fluorescence units; m/z, mass/charge ratio; MS, mass spectrometry; MSn, tandem mass spectrometry; MAGS, Metadata Assisted Glycan Sequencing. (B) Biotinylated lectins were bound to the HL-SGM at the noted concentrations and were detected with cyanine 5–conjugated streptavidin. ConA, concanavalin A; WGA, wheat germ agglutinin; RCA, R. communis agglutinin; AAL, A. aurantia lectin; ECL, E. cristagalli lectin; LEL, L. esculentum lectin.