Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 13;5(2):eaav2554. doi: 10.1126/sciadv.aav2554

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.

PMC Copyright notice

Fig. 3 — (A) NA treatment of HL-SGM and interrogation with SNA (25 μg/ml; detected with cyanine 5–conjugated streptavidin) and Penn reveal that IAV binds to array after removal of Sia as determined by loss of SNA binding. (B) Matrix-assisted laser desorption/ionization–time-of-flight MS (MALDI-TOF-MS) analysis of fractions, R10N13 (chart ID 118; high binding) indicating sialylated N-glycans and R04N23 (chart ID 47; high binding after NA treatment) indicating phosphorylated glycans, and tandem MS (MS/MS) of selected peaks. (C) Neuraminidase treatment of highest binding fraction R10N13 (predicted structures shown to the left; blue square, N-acetylglucosamine; red triangle, fucose; green circle, mannose; yellow circle, galactose; purple diamond, Sia) and binding of SNA (red bars) and MAL-I (blue bars) reveal the presence of both linkage types within the fraction.