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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Methods Cell Biol. 2018 May 1;144:185–231. doi: 10.1016/bs.mcb.2018.03.008

Figure 11. Signal depth correction.

Figure 11.

Schematic illustrating method used for depth correction of fluorescence signal intensity. GFP::GSP-2 signal correlates with mCh::H2b signal, thus intensity changes in GFP signal due to variation in distance between the objective and object can be corrected ratio to mCh signal.