Skip to main content
. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Methods Cell Biol. 2018 May 1;144:185–231. doi: 10.1016/bs.mcb.2018.03.008

Figure 2. Methods for fluorescently tagging proteins in situ using CRISPRCas9.

Figure 2.

(A) Schematic illustrating the CRISPR-Cas9 method (see text for details). Illustrations describing two methods for in situ tagging, using either direct integration (B) or the self-excising cassette method (C). (D) Plasmids to be generated for CRISPR/Cas9-mediated in situ tagging.