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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Methods Cell Biol. 2018 May 1;144:185–231. doi: 10.1016/bs.mcb.2018.03.008

Figure 3. Generation of gene replacement systems using RNAi-resistant transgenes.

Figure 3.

Described here is the strategy used for the generation of an RNAi-resistant transgene for CDC-20 (also known as FZY-1). (A) Schematic illustrating the strategy for single-copy transgene insertions using MosSCI. The CDC-20 transgene was inserted in chromosome I and rendered resistant to an RNAi targeting its coding sequence (B) Analysis of transgene expression through immunoblot. A control (N2) sample was titrated in order to assess depletion efficiency and rescue of CDC-20 expression. (C) Rescue of embryonic lethality by the RNAi-resistant CDC-20 transgene. (Adapted from Kim et al. 2017)