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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Methods Cell Biol. 2018 May 1;144:185–231. doi: 10.1016/bs.mcb.2018.03.008

Figure 6. Quantification of nuclear reformation and centriole assembly.

Figure 6.

(A) Schematics illustrating two early events in the C. elegans embryo: nuclear reformation after exit from meiosis II and centriole assembly. Parameters such as p=pronuclear diameter and c=centriole intensity can be measured over time. (B) Measuring male pronuclear diameter in either control embryos or embryos depleted of the nucleoporin mel-28 (Adapted from Hattersley et al. 2016). (C) Measuring SAS6 accumulation at centrioles upon centriole assembly. Embryos depleted of endogenous SAS-6 by RNAi were expressing a reencoded version of SAS-6::GFP, either wild-type or a mutant deficient in centriole assembly (I154E) and centriole intensities were quantified over time (adapted from Lettman et al. 2013)