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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Methods Cell Biol. 2018 May 1;144:185–231. doi: 10.1016/bs.mcb.2018.03.008

Table 1.

Oligonucleotides used for the identification of MosSCI integration strains by PCR.

Oligonucleotides Expected
product		 Notes
Forward Reverse
Mos I (ttTi4348);
left integration		 cgtcagagaggagagg
aacg		 aaattcaggtctgtacgg
aagc		 2093 bp
Mos I (ttTi4348);
right integration		 cattcgaagatctgccc
act		 gagtccaacgcttatttct
cg		 1754 bp
Mos II (ttTi5605);
left integration		 cgtcagagaggagagg
aacg		 gacatttgagaatggcat
tga		 1828 bp Also for Uni-MosSCI
integrations
Mos II (ttTi5605);
right integration		 cattcgaagatctgccc
act		 atcgggaggcgaacct
aactg		 1524 bp Also for Uni-MosSCI
integrations