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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Methods Cell Biol. 2018 May 1;144:185–231. doi: 10.1016/bs.mcb.2018.03.008

Table 2.

Oligonucleotides used for the genotyping of MosSCI integration strains by PCR.

Ooligonucleotides Expected product Notes
Forward Reverse Internal Wild-type Integrant
Mos I
(ttTi4348)		 cccaaaatac
ctcccctcat		 tccggagaaa
aacttccaaa		 gcgatctttgaa
gaagtgacg		 544 bp 1680 bp
Mos II
(ttTi5605)		 cgaaatgtcct
cctgattcc		 cgccattgtttc
ctgaaaat		 gcgatctttgaa
gaagtgacg		 654 bp 1620 bp
Uni I
(oxTi185)		 gttccatagcc
accatcacc		 gcatcgctaaa
aacgagagg		 tatcgtaaatcg
gcgcgagc		 914 bp 1242 bp Use a 62°C annealing
temperature protocol
Uni IV
(oxTi177)		 gtcactcaaac
cgatgcaga		 gcaatttcggc
aatttcagt		 tatcgtaaatcg
gcgcgagc		 324 bp 566 bp Use a 62°C annealing
temperature protocol
Uni V
(oxTi365)		 ccctcttttggg
ctctgcaa		 ttgatgaaagg
caagcgtgaa		 tatcgtaaatcg
gcgcgagc		 1083 bp 1316 bp Use a 62°C annealing
temperature protocol