. Author manuscript; available in PMC: 2020 Apr 1.

Published in final edited form as: J Med Virol. 2018 Oct 31;91(4):677–686. doi: 10.1002/jmv.25339

Table 3.

Neutralizing capability of selected bovine serum samples from calves on the infectivity of a genotype 3 human HEV (Kernow P6 strain) in HepG2/C3A human liver cells

Bovine Serum ID and controls	Date of sample collection	ELISA O.D. value	Individual S/C.O. value	% Decrease in HEV infectivity^a	Sample neutralizing antibody titer
PBS	N/A	N/A	N/A	0	N/A
Chimp 1313	N/A	N/A	N/A	91.87	N/A
5078	1/14/13	0.411	2.658	48.56	1:16
5082	12/5/12	0.340	2.270	46.9	1:4
5082	1/10/13	3.125	20.851	49.28	1:16

All bovine sera were tested in dilutions 1:10, 1:100 and 1:1000, and were also titered in serial dilutions from 1:2 to 1:8192 in a serum virus neutralization assay. Chimp 1313 is a hyperimmune HEV antisera as a positive control with at least 10⁵ IgG ELISA titer against HEV from a chimpanzee experimentally-infected with HEV.