Figure 4:

(A) Anesthesized SS rats were infused renal intersititially with PP242 at the dose of 5mg/kg (black circle, n=6) or vehicle (open circle, n=6), and urine was collected at 15 minute intervals. The average mean value Na⁺ excretion rate and urine volume determined in these rats. (B) Na⁺ excretion rate of i.v. infusion of PP242 with dose 10 ug/kg/min (black circle, n=3) or rapamycin (10 ug/kg/min; black triangle, n=4) or vehicle (open circle, n=3) was determined in SS rats. (C) Sodium balance in chronic administration of PP242 i.p. 15mg/kg/day (black circle, n=5) or vehicle (open circle, n=6) to SS rats. Rats were fed a gel diet delivering a fixed sodium intake per day. In the control period rats were not receiving PP242 or vehicle. Rats were treated with PP242 or vehicle while maintaining at 0.4% NaCl diet for 4 days before switching to 4% NaCl diet for 5 days. * P<0.05 vs Vehicle.