Figure 6:

. (A&B) SS rats were injected with 15 mg/kg PP242 or vehicle and kidneys were harvested after 1 hr and 24 hr of treatment, respectively. Western blots of pAKT^S473 and pS6^S235/236 were normalized with total endogenous AKT and S6 proteins, respectively. (C) To determine inhibitory effect of PP242 on surrogate marker of SGK1 (pNDRG1^T346/NDRG1), kidneys were excised 1 hr post-IP injection. Medullary tissue was used to asses pAKT^T308, pAKT^S473 and pNDRG1^T346 as well as AKT, NDRG1 and GAPDH expression by Western blot analysis. In all panel graphs represent the fold change of pAKT^S473/AKT or pS6^S235/236/S6 or pNDRG1^T346/NDRG1 or pAKT^T308/AKT from vehicle in the cortex or medulla of these rats. Student’s t-test was used to compare PP242 and vehicle treatment.