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. 2019 Feb 7;10:86. doi: 10.3389/fmicb.2019.00086

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Copyright © 2019 Tams, Cassilly, Anaokar, Brewer, Dinsmore, Chen, Patton-Vogt and Reynolds.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Biosynthesis of both PC and PE via the Kennedy pathway requires EPT1. (A) Membranes were isolated from all strains and used in an in vitro PC synthesis assay, where ^[14C]CDP-choline incorporation into PC was measured. Enzyme activity was measured as counts per minute per milligram of protein, and is expressed as a percentage of wild-type (^∗∗p < 0.01; unpaired t-test compared to wild-type, WT). (B) Each strain was cultured in the presence of ^[14C]ethanolamine and incorporation of the radiolabel into PE was quantified by phosphorimager from thin-layer chromatography (^∗p < 0.05; compared to wild-type, WT, by the unpaired t-test). Each of these assays was performed with three biological replicates.