Table 1.
Node | Divergence event | Estimated age ± SD (95% HPD confidence interval) | ||
---|---|---|---|---|
Best-fit models (Analysis A) | HKY model (Analysis B) | Average age | ||
I | Split of Microhylinae from Dyscophinae | 67.3 (62.1–72.6) | 66.6 (61.6–71.6) | 67.0 (K-Pg boundary) |
II^ | Spilt between (Glyphoglossus + Microhyla + Micryletta + Mysticellus) and (Kaloula + Metaphrynella + Phrynella + Uperodon) | 61.9 (57.2–66.5) | 61.0 (56.6–65.5) | 61.5 (Paleocene) |
III^ | Spilt between (Glyphoglossus + Microhyla) and (Micryletta + Mysticellus) | 57.6 (52.3–62.6) | 58.0 (51.7–60.9) | 57.8 (Paleocene/Eocene) |
IV* | Spilt between Glyphoglossus and Microhyla | 48.1 (44.1–52.2) | 49.2 (45.1–53.2) | 48.7 (Eocene) |
V* | Spilt between two major Microhyla clades | 44.6 (38.2–48.3) | 45.5 (39.6–48.7) | 45.1 (Eocene) |
VI ** | Split between Micryletta and Mysticellus | 40.6 (29.1–51.4) | 38.8 (29.5–48.1) | 39.7 (Eocene) |
VII** | Split between Uperodon and (Kaloula + Metaphrynella + Phrynella) | 38.8 (30.6–47.3) | 42.7 (35.8–49.8) | 40.8 (Eocene/Oligocene) |
VIII^ | Split between Kaloula and (Metaphrynella + Phrynella) | 34.6 (26.6–42.8) | 37.8 (30.8–44.8) | 36.2 (Eocene/Oligocene) |
IX^ | Split between Metaphrynella and Phrynella | 19.2 (12.9–25.8) | 23.3 (17.1–29.9) | 21.3 (Oligocene/Miocene) |
Ages are in Million years; HPD = highest posterior density. Best-fit models are provided in Supplementary Table S4. *Denotes first postulated Miocene exchange; **Denotes second postulated Miocene exchange; ^Denotes diversification events associated with periods of isolation.