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. 2019 Feb 13;9:1906. doi: 10.1038/s41598-018-38133-x

Table 1.

Estimated divergence ages of major microhyline lineages.

Node Divergence event Estimated age ± SD (95% HPD confidence interval)
Best-fit models (Analysis A) HKY model (Analysis B) Average age
I Split of Microhylinae from Dyscophinae 67.3 (62.1–72.6) 66.6 (61.6–71.6) 67.0 (K-Pg boundary)
II^ Spilt between (Glyphoglossus + Microhyla + Micryletta + Mysticellus) and (Kaloula + Metaphrynella + Phrynella + Uperodon) 61.9 (57.2–66.5) 61.0 (56.6–65.5) 61.5 (Paleocene)
III^ Spilt between (Glyphoglossus + Microhyla) and (Micryletta + Mysticellus) 57.6 (52.3–62.6) 58.0 (51.7–60.9) 57.8 (Paleocene/Eocene)
IV* Spilt between Glyphoglossus and Microhyla 48.1 (44.1–52.2) 49.2 (45.1–53.2) 48.7 (Eocene)
V* Spilt between two major Microhyla clades 44.6 (38.2–48.3) 45.5 (39.6–48.7) 45.1 (Eocene)
VI ** Split between Micryletta and Mysticellus 40.6 (29.1–51.4) 38.8 (29.5–48.1) 39.7 (Eocene)
VII** Split between Uperodon and (Kaloula + Metaphrynella + Phrynella) 38.8 (30.6–47.3) 42.7 (35.8–49.8) 40.8 (Eocene/Oligocene)
VIII^ Split between Kaloula and (Metaphrynella + Phrynella) 34.6 (26.6–42.8) 37.8 (30.8–44.8) 36.2 (Eocene/Oligocene)
IX^ Split between Metaphrynella and Phrynella 19.2 (12.9–25.8) 23.3 (17.1–29.9) 21.3 (Oligocene/Miocene)

Ages are in Million years; HPD = highest posterior density. Best-fit models are provided in Supplementary Table S4. *Denotes first postulated Miocene exchange; **Denotes second postulated Miocene exchange; ^Denotes diversification events associated with periods of isolation.