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. 2019 Feb 13;9:1971. doi: 10.1038/s41598-018-37192-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of indels at the transgene integration sites. The PCR-specific amplicons as shown in Fig. 3 were gel extracted and analyzed by Sanger sequencing. (A) Indels created at the non-targeted ROSA26 locus for the indicated founder animals. (B,C) Indels created at the targeted ROSA26 locus for (B) forward and (C) reverse integration events. Wild-type and donor sequences are shown at the top with the targeting gRNA site underlined and protospacer adjacent motif (PAM) sequences bolded. The dash lines denote deletions (Δ) and insertions (ins) with the number of nucleotides indicated. Italics font denotes mutations at the indel site.