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. 2019 Feb 13;9:1971. doi: 10.1038/s41598-018-37192-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Global expression of the encoded tdTomato transgene in ROSA26-targeted (ROSA-TG) ferrets. (A) Representative en face fluorescent images of the indicated whole mount organs in the transgenic F1 newborn ferret (A1) demonstrate ubiquitous expression of the tdTomato transgene. (B,C) Fluorescent photomicrographs of phalloidin-stained (green) cryosections for brain cortex, cardiac muscle, liver hepatocytes, skeletal muscle and spleen from a newborn (B) non-transgenic and (C) transgenic ferret (A1). Phalloidin was used for staining filamentous actin (F-actin) to allow for better visualization of tissue structure. DAPI was used to stain nuclei.