Table 1.

The efficiency and outcome of Cas9-mediated knock-in at the ferret ROSA26 locus.

Experiment	Zygotes Injected	Zygotes Transferred	Births	tdTomato Positive	ROSA Insertion	Random Insertion	Orientation (F/R)
A	21	18	2	2	2 (A1, A3)	0	F-A1; R-A3
B	37	34	6	2	2 (B2, B5)	2 (B2, B5)	R-B2; F-B5
C	45	39	5	0	—	—	—
D	41	33	4	1	1 (D1)	0	F-D1
E	35	27	6	1	0	1 (E1)	NA
Total	179	151	23	6	5	3	F-3 R-2

F, forward; NA, unidentified; R, reverse.