. 2019 Feb 13;9:1971. doi: 10.1038/s41598-018-37192-4

Table 2.

Sequences of primer sets and conditions used for genotyping.

Inserted orientation	Primer ID (F/R)	Primer pair ID	Sequence (5′→3′)	Tm (°C)	Product size (bp)
Wild type	W-F	P1	CCTAACCAAAGGGATGCT	56	900
Wild type	W-R	P1	AATGAGCGAAACCACTGAG	56	900
Forward insertion	FU-F	P2	GGAGGAATAAATACATAACTGG	57	1030
	FU-R	P2	ACTGACGGTCGTAAGCAC	57	1030
	FD-F	P3	TGTTTCAGTGGGTATGGC	57	1013
	FD-R	P3	GTTTGTTAGGCTGGAAGG	57	1013
Reverse insertion	RU-F	P4	CAATCACTCTGCCTTTAACCTC	59	931
	RU-R	P4	TGGGCTCTATGGCTTCTG	59	931
	RD-F	P5	ATGAACTAATGACCCCGTAA	60	621
	RD-R	P5	CCAAATGAGCGAAACCAC	60	621

bp, base pair; FD, forward downstream; FU, forward upstream; RD, reverse downstream; RU, reverse upstream; Tm, annealing temperature; W, wild type.