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. 2019 Feb 13;9:1913. doi: 10.1038/s41598-018-38315-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Phospho-RTK arrays of HCB-514 were done at basal conditions (up) and upon EGF-stimulation (down). Each RTK is represented in duplicate in the arrays (two spots side-by-side), and three pairs of phospho-tyrosine positive controls are located in the corners of each array. (b) Western blot of EGFR phosphorylation, showing the different amount of phospho-EGFR and total EGFR compared to the SiHa cell line. Alpha-tubulin was used as endogenous control. (c) Quantification of phospho-EGFR normalized with total EGFR.