Fig. 5.

TMEM33 is required for VEGFA-mediated angiogenesis in HUVECs. a TMEM33 expression is significantly reduced by small interfering RNA (siRNA) knockdown. b Representative images of wound-healing migration assays performed with HUVECs transfected with a non-targeting control siRNA (siRNA-NT) or a siRNA specific for human TMEM33 (siRNA-TMEM33) after incubation in culture medium supplemented with VEGF (25 ng/ml). Data are shown as mean ± SE, n = 9, obtained from three independent experiments for comparison of cells transfected with siRNA-TMEM33 vs. siRNA-NT (unpaired two-tailed, Student’s t-test ****p ≤ 0.001). Images were taken at time zero and after incubation for 24 h. The migrated area was calculated by subtracting the value of the non-migrated area from the wound area at time zero and expressing this as a percentage of the total area at time zero. Scale bars, 1000 µm. c HUVECs transfected as described in a were plated on Growth-Factor Reduced Matrix (Geltrex) in Medium 200 containing 2% FBS (Control), supplemented with VEGF (25 ng/ml) when indicated (VEGF). Images show representative fields from experiments quantified in the histogram. Data are shown as mean ± SE, n = 8, obtained from three independent experiments; ns = nonsignificant; **p ≤ 0.01; ***one-way ANOVA with post-hoc Tukey’s comparison test p ≤ 0.005. Scale bars, 1000 µm. Source data are provided as a Source Data file