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. 2019 Feb 13;9:1920. doi: 10.1038/s41598-019-38566-y

Figure 4.

Figure 4

Anti-miR-17 improves renal function and reduces kidney injury and cyst proliferation. (A) Blood urea nitrogen (BUN) was decreased by 23% in anti-miR-17 treated mice compared to PBS treated mice. However this difference was not statistically significant. (B,C) Q-PCR analysis revealed that expression of kidney injury markers, Kim1 and Ngal was also reduced only in kidneys of anti-miR-17-treated mice. (N = 6 per group) (D,E) To assess proliferation, kidney sections were stained using an antibody against phosphohistone-H3 (pHh3), a marker of proliferating cells. Quantification of PHh3 positive cells from ten random high-powered images (20×) from each kidney section revealed that only anti-miR-17-treated mice showed a reduction in the number of proliferating cyst cells. Data are presented as mean ± SEM. Statistical analyses: One-way ANOVA (post hoc analysis: Dunnett’s multiple comparisons test), ns indicates P > 0.05.