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. 2019 Feb 13;9:1997. doi: 10.1038/s41598-018-38289-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RarA-YFP characterization (A) Chronic viability assay for the RarA-YFP expressing strain. The presence of the YFP tag does not affect the viability of the strain; (B) chronic viability of ∆recX/RarA-YFP compared to wild type and ∆recX mutant. The chronic exposure to MMS produces the death of both mutant strains in the same way while wild type is still surviving; (C) Thermosensitivity assays for dnaB37 and dnaC30 replication mutants expressing RarA-YFP. The fluorescence tag does not affect the response of any of the single mutants; (D) RarA-YFP foci in B. subtilis BG1331 (rarA-yfp) cells during exponential growth after 700 ms exposure to 515 nm laser excitation. Only ~15% of the cells contain foci.