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. 2019 Feb 13;9:1997. doi: 10.1038/s41598-018-38289-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RarA interactions with components of the replication machinery. (A) Percentage of cells that contains RarA-YFP foci in thermosensitive replication mutants compared to wild type cells in epifluorescence microscopy, during exponential growth at 25 °C (OD₆₀₀ = ~0.3) and after addition of MMC (50 ng /ml) for 60 min or swift to non-permissive conditions (42 °C). Error bars shows standard deviation of at least three independent experiments; (B) Weight of different DnaX-CFP and RarA-YFP colocalization patterns after addition of H₂O₂ (1 mM) or MMC (50 ng/ml) for 60 min compared to the control without drug. The percentage of not-merged foci is split at the right of the panel into two different localization patterns: near and far. (C) Examples of the colocalization of DnaX-CFP and RarA-YFP (up) and non-colocalizing patterns defined as near (medium) and far (down). (D) RarA-mVenus single-molecule DPD in thermosensitive mutants. Swift to non-permissive temperature (42 °C) leads to drastic changes in dynamics in absence of external DNA damage, and alters the normal response of RarA after addition of H₂O₂ or MMC.