Table 2.
Background | D (μm2 s−1) | 25 °C −drug | 42 °C −drug | 42 °C+H2O2 | 42 °C+MMC |
---|---|---|---|---|---|
static dyna | static dyn | static dyn | static dyn | static dyn | |
Wild type | 0.23 2.3 | 48 52 | 45 55 | ND ND | ND ND |
dnaB37 | 0.2 1.8 | 41 59 | 65 35 | 40 60 | 58 42 |
dnaC30 | 0.19 2.0 | 38 62 | 29 71 | 38 62 | 48 52 |
Apparent diffusion coefficient (in μm2 s−1) and population weights (in %) calculated by step-size distribution and Gaussian fit for RarA-mVenus in the replication deficient thermosensitive mutants studied in exponential growth for 60 min at permissive (25 °C) and non-permissive (42 °C) temperature in the presence or absence of H2O2 (0.5 mM) or MMC (50 ng/ml). At least 200 tracks/condition were considered for the analysis. adyn, dynamic; ND, not done.