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. 2019 Feb 13;9:1895. doi: 10.1038/s41598-018-38382-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Biosynthetic pathway of ERG in M. smegmatis. ERG biosynthesis is mediated by five enzymatic steps. Expression of the foreign genes for EgtB, EgtC, EgtD and EgtE allows E. coli cells to synthesize ERG. EgtA from M. smegmatis is not indispensable because E. coli possesses GshA that catalyzes formation of γ-GC from Cys and glutamate. Met is used following formation of SAM by SAM synthetase (MetK in E. coli).