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. 2019 Feb 13;9:1895. doi: 10.1038/s41598-018-38382-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of pCys^HP and egtA is remarkable efficient in ERG productivity. WT and CH harboring each of plasmids (pQE1a, pQE1a-egtBCDE, pQE1a-egtABCDE) were cultured in SM1 liquid medium. After 6 h cultivation, IPTG and Na₂S₂O₃ were added at concentrations of 0.1 mM and 10 mM respectively. Data are presented as mean values with standard errors from three independent experiments. pACYC184 as negative control for pCys^HP was introduced into WT.