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. 2019 Feb 13;9:1895. doi: 10.1038/s41598-018-38382-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Impact of metJ gene disruption on ERG and HER productivities. (A) CH pQE1a-egtABCDE and CH ΔmetJ pQE1a-egtABCDE were cultured in SM1 liquid medium. After 6 h cultivation, IPTG and Na₂S₂O₃ were added at concentrations of 0.1 mM and 10 mM respectively. Cell density (left) was estimated from the OD at 562 nm. ERG (right) in the cultured supernatant at 120 h was quantified by Sulfur index analysis described in Methods. (B) WT and ΔmetJ cells harboring pCys^HP and pCF1s-egtD were cultured in SM1 liquid medium. After 6 h cultivation, IPTG and Na₂S₂O₃ were added at concentrations of 0.1 mM and 10 mM respectively. HER in the cultured supernatant at 120 h was quantified by LC-MS/MS analysis. Data are presented as mean values with standard errors from three independent experiments.