Table 1.
Details of the samples used in this study.
| Region | Sampling site | Code | N | Depth (m) | Coordinates | MLG | 1-MLG/N (%) Percentage of shared MLGs among samples | N. of Green lineage | N. of blue/red lineage (Fig. 2) | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| recently colonized in temperate area | Goto Islands | Oushima | GAHo | 19 | 0.3~6.0 | 32°33′ 55.47″ N | 128°53′ 56.57″ E | 9 | 0.526 | 9 | 0 |
| Kuroshima | GAHk | 13 | 4.1~5.6 | 32°35′ 56.18″ N | 128°50′ 04.05″ E | 10 | 0.231 | 10 | 0 | ||
| Kumamoto | Amakusa | AMK | 25 | N.A. | 32°33′ 16.16″ N | 129°59′ 59.91″ E | 23 | 0.080 | 23 | 0 | |
| Izu Islands | Shikinejima | ZAH | 18 | 0.8~6.6 | 34°21′ 36.44″ N | 139°12′ 02.39″ E | 18 | 0.000 | 17 | 1 | |
| pre-existing temperate area | Wakayama | Kushimoto | WAHk | 34 | 2.4~5.5 | 33°28′ 27.36″ N | 135°44′ 26.92″ E | 29 | 0.147 | 29 | 0 |
| Shirahama | WAHs | 18 | 0.7~9.4 | 33°40′ 59.81″ N | 135°20′ 11.41″ E | 18 | 0.000 | 18 | 0 | ||
| Kochi | Tatsukushi | KOC | 32 | 1.0~6.0 | 32°45′ 49.33″ N | 132°51′ 38.39″ E | 31 | 0.031 | 30 | 1 | |
| Miyazaki | Tsukijima | TUK | 46 | N.A. | 31°28′ 39.18″ N | 131°23′ 14.67″ E | 30 | 0.348 | 23 | 7 | |
| Toimisaki | TOI | 29 | N.A. | 31°21′ 00.47″ N | 131°16′ 59.92″ E | 28 | 0.034 | 25 | 3 | ||
| Yakushima | Tsukasaki | KAHyn | 30 | 4.4~6.3 | 30°25′ 55.50″ N | 130°34′ 34.46″ E | 29 | 0.033 | 24 | 5 | |
| Nanase | KAHys | 13 | 5.6~7.7 | 30°14′ 42.24″ N | 130°25′ 31.24″ E | 9 | 0.357 | 8 | 1 | ||
| subtropical area | Amami Islands | Saneku | KAHaw | 22 | 1.5~4.8 | 28°11′ 33.24″ N | 129°11′ 30.78″ E | 22 | 0.000 | 0 | 22 |
| Sakibaru | KAHae | 16 | N.A. | 28°19′ 54.83″ N | 129°33′ 19.76″ E | 16 | 0.000 | 14 | 2 | ||
| Okinawa Island | Sesokojima | SSK | 35 | N.A. | 26°37′ 51.43″ N | 127°51′ 23.57″ E | 35 | 0.000 | 0 | 35 | |
| Oura | OAH | 42 | N.A. | 26°30′ 21.70″ N | 128°02′ 28.84″ E | 42 | 0.000 | 9 | 33 | ||
| Miyakojima | Yoshino | MYK | 47 | N.A. | 24°47′ 57.72″ N | 125°20′ 59.22″ E | 47 | 0.000 | 9 | 38 | |
| Sekisei Lagoon | Uro | SAHaaf | 36 | 1.8~4.2 | 24°15′ 28.63″ N | 124°10′ 39.49″ E | 36 | 0.000 | 0 | 36 | |
| Hanari East | SAHd | 49 | 1.0~6.6 | 24°13′ 20.22″ N | 123°56′ 16.41″ E | 48 | 0.020 | 1 | 47 | ||
| Yonara Chanel | SAHfqy | 65 | 1.0~3.8 | 24°22′ 00.32″ N | 123°57′ 03.14″ E | 65 | 0.000 | 0 | 65 | ||
| Kohamajima East | SAHk | 44 | 0.5~2.8 | 24°21′ 06.98″ N | 124°00′ 41.96″ E | 44 | 0.000 | 0 | 44 | ||
| Haimi South | SAHt | 28 | 2.2~4.8 | 24°16′ 06.23″ N | 123°50′ 06.66″ E | 28 | 0.000 | 1 | 27 | ||
| Aragusuku East | SAHae | 28 | 2.8~4.7 | 24°15′ 07.03″ N | 123°58′ 25.77″ E | 28 | 0.000 | 2 | 26 | ||
The numbers of samples used in the population genetic analysis are shown in bold. N, total number of samples collected from each site. MLG, number of multilocus genotypes. N. of green lineage, number of green lineage samples; N. of blue/red lineage, number of blue/red lineage samples (Fig. 2).
N.A. not available because of the lack of depth data