Fig. 1.

PIN1 dynamic polarization pattern during 12 h growth after 5 mM LaCl₃ treatment. a, c PIN1-GFP signal indicates a new primordium forming after 12 h (dashed square). b, d Enlargement of the square region in a, c. Colored dots indicate the cells that had PIN1-GFP polarity changed over 12 h of growth. e–g PIN1-GFP dynamic polarization pattern freezes after repeated application of 5 mM LaCl₃ for 2 min in every hour for 12 h without sample rinse (f), but primordia resume growth and PIN1-GFP signal relocalizes in the dashed square region after 12 h of recovery following water rinse (g). h–j Enlargement of the square region in e–g. The cells that are labeled by colored dots show that PIN1-GFP does not repolarizes during LaCl₃ treatment but resumes a dynamic pattern after a 12 h recovery. a–j scale bar: 20 µm. LUT is gem in Fiji-ImageJ software