Fig. 4.

The PIN1 mechanical response is initiated within 5 min of stimulus and is not reversed by subsequent Ca²⁺ inhibitor treatments. a, b PIN1-GFP behaves similarly to an untreated control at 3 h when pipette-mediated cell ablation precedes (by 5 min) the application of 5 mM LaCl₃ (which lasts 15 min with no subsequent washout). c, d PIN1-GFP behaves similarly to an untreated control at 3 h when pipette-mediated cell ablation precedes (by 5 min) the application of 2 mM BAPTA (which lasts 10 min with no subsequent washout), as in a, b. a–d, Scale bar: 20 µm. LUT is gem in Fiji-ImageJ software