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. 2019 Feb 13;9:1974. doi: 10.1038/s41598-019-39122-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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EZH2 functions through its target genes. (A,B) Diagram shows the mRNA expression of EZH2 and its target genes in control and DZNepA treated MCF-7 and MDA-MB-231 breast cancer cells respectively observed in reverse transcriptase PCR. (C) Reverse transcriptase PCR data shows expression of EZH2 and its target genes in xenograft collected from vehicle, nicotine and nicotine & DZNepA treated mice. (D) [i] Expression of SUMF1 in tissue sections from smoking and non-smoking breast cancer patients as observed in immunohistochemistry. [ii] Scatter plot depicts the percent SUMF1 positive cancer cells in the tissue section from smoking and non-smoking patient samples. [iii] Scatter plot shows the intensity score of SUMF1 expression in two experimental groups. [iv] Graph displays the Q-score of SUMF1 expression in experimental groups. Two tailed paired Student t-test was used for statistical analysis *P < 0.05.