Figure 2.

Optimization of the reagent concentration and time of maximum reactivity for aqeous and methanolic ABTS, DPPH, and PPR leaf disc assays. (A–C) Shows the time-dependent decay of ABTS^•+ radical by various leaf discs at different reagent dilution ratios, 1:30 (A), 1:40 (B) and 1:50 (C) with water at wavelength 725 nm. (D–F) Indicates time-dependent scavenging of DPPH radical by different leaf discs in a methanolic DPPH solution of concentrations, 0.204 (D), 0.102 (E) and 0.051 mM (F) at wavelength 515 nm. The various leaf discs-based reduction of a KMnO₄ solution of different concentrations, 0.500 (G), 0.250 (H) and 0.125 mM (I) at wavelength 525 nm. The value at each time point represents the mean absorbance of technical replicates (n = 3–6).