Table 2.
Identification of phenolic compounds from various leaf materials by UHPLC/ESI‐HR‐QTOFMS.
| Leaf material | Rt (min) | UV λmax (nm) | Experi-mental mass (m/z) | MS/MS fragments | Compound | Molecular formula | Theoretical mass (m/z) | Mass error (ppm) | ref. |
|---|---|---|---|---|---|---|---|---|---|
| Spinach | 11.8 | 255, 350 | 789.2270 | 333 | Patuletin-3-O-β-D-glucopyranosyl-(1 → 6)-[β-D-apiofuranosyl-(1 → 2)]-β-D-glucopyranoside | C33H40O22 | 789.2083 | −9.5 | 25 |
| 12.3 | 255, 350 | 657.1630 | 347, 333 | Patuletin-3-O-β-D-glucopyranosyl-(1 → 6)-β-Dglucopyranoside | C28H32O18 | 657.1661 | −4.7 | 25 | |
| 12.8 | 255, 350 | 803.2190 | 421, 347 | Spinacetin-3-O-β-D-glucopyranosyl-(1 → 6)-[β-D-apiofuranosyl-(1 → 2)]-β-D-glucopyranoside | C34H42O22 | 803.2240 | −6.3 | 25 | |
| 18.0 | 254, 278, 340 | 521.0931 | 345, 330 | 5, 3, 4 -Trihydroxy-3-methoxy-6:7-methylendioxyflavone-4 - β-D-glucuronide | C23H20O14 | 521.0926 | 1.0 | 25 | |
| Kale | 8.4 | 254, 340 | 789.2081 | 425, 347, 303, 204, 132, 84 | Quercetin-3-diglucoside-7-glucoside | C33H40O22 | 789.2084 | −0.4 | 22, 23 |
| 8.9 | 258, 338 | 773.2136 | 347, 287, 186, 132, 84 | Kaempferol-3-diglucoside-7-glucoside | C33H40O21 | 773.2135 | 0.1 | 22, 23 | |
| 9.2 | 258, 338 | 934.2633 | 633, 571, 449, 347, 287, 84 | Kaempferol-3-diglucoside-7-diglucoside | C39H50O26 | 934.2585 | 5.2 | 22, 23 | |
| Collards | 8.9 | 268, 344 | 773.2206 | 541, 347, 287 | Kaempferol-3-diglucoside-7-glucoside | C33H40O21 | 773.2135 | 9.2 | 21, 22 |
| 9.5 | 253, 268, 338 | 965.2561 | 449, 355, 287, 193, 133, 84 | Kaempferol-3-hydroxyferuloyldiglucoside-7-glucoside | C43H48O25 | 965.2557 | 0.4 | 21, 22 | |
| 9.7 | 338 | 935.2561 | 409, 287, 163, 84 | Kaempferol-3-caffeoyldiglucoside-7-glucoside | C42H46O24 | 935.2451 | 11.7 | 21, 22 | |
| 10.1 | 338 | 965.2590 | 409, 303 | Quercetin-3-O-feruloyldiglucoside-7-glucoside | C43H48O25 | 965.2557 | 3.4 | 21, 22 | |
| 10.5 | 254, 268, 334 | 1111.3239 | 949, 532, 449, 287, 177, 84 | Kaempferol-3-feruloyltriglucoside-7-glucoside | C49H58O29 | 1111.313 | 9.2 | 21, 22 | |
| Mustard | 8.9 | 268, 344 | 773.2206 | 541, 347, 287 | Kaempferol-3-diglucoside-7-glucoside | C33H40O21 | 773.2135 | 9.2 | 21, 22 |
| 9.5 | 253, 268, 338 | 965.2625 | 449, 355, 287, 193, 161, 133, 84 | Kaempferol-3-hydroxyferuloyldiglucoside-7-diglucoside | C43H48O25 | 965.2557 | 7.0 | 21, 22 | |
| 9.7 | 253, 268, 338 | 935.2463 | 409, 287, 163, 135, 84 | Kaempferol-3-caffeoyldiglucoside-O-glucoside | C42H46O24 | 935.2451 | 1.2 | 21, 22 | |
| 10.1 | 253, 338 | 965.2590 | 409, 303 | Quercetin-3-O-feruloyldiglucoside-7-O-glucoside | C43H48O25 | 965.2557 | 3.4 | 21, 22 | |
| 10.5 | 253, 268, 334 | 611.1577 | 287 | Kaempferol-3-diglucoside | C27H30O16 | 611.1606 | −4.8 | 21, 22 | |
| 10.8 | 268, 338 | 641.1695 | 317 | Isorhamnetin-3-diglucoside | C28H32O17 | 641.1712 | −2.7 | 21, 22 | |
| 14.8 | 254, 266, 352 | 479.1155 | 317 | Isorhamnetin-3-glucoside | C22H22O12 | 479.1184 | −6.1 | 21, 22 | |
| Watermelon | 7.5 | 246, 330 | 707.2320 | 365, 163, 89 | Unknown | — | — | — | — |
| 8.5 | n.d. | 827.3534 | 425, 319, 157, 107 | Unknown | — | — | — | — | |
| 15.7 | 250, 332 | 587.2183 | 319, 163, 107, 89 | Unknown | — | — | — | — |
n.d.- indicates not detecte.