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. 2019 Feb 13;9:1956. doi: 10.1038/s41598-018-38471-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Misalignment of osteoblasts in Tnfsf11^−/− mice. Areas shown in all panels correspond to the boxed area in the basioccipital bone in Fig. 1e. (a) TRAP staining in representative sections of WT (n = 3) and Tnfsf11^−/− (n = 2) mice at P0. Arrows, TRAP-positive osteoclasts at the dorsal periosteum and endosteum. Scale bars, 100 μm. (b) Osteocalcin-positive osteoblasts (arrowheads) in representative sections of WT and Tnfsf11^−/− mice at P0. Scale bars, 100 μm. (c–e) Alizarin complexone (c, injected at P3) and calcein (d, injected at P4) double-labelling (e, merged image) in representative sections of WT (n = 3) and Tnfsf11^−/− (n = 2) mice at P5. Scale bars, 200 μm.