Table 1.
miR-672-5p Improves micro-CT Parameters and Biomechanical Properties in Vertebra of Mice
| Parameters | PBS | miR-C | miR-672-5p | PBS | miR-C | miR-672-5p |
|---|---|---|---|---|---|---|
| Sham | OVx | |||||
| vBMD (gHA/cm3) | 0.317 ± 0.007 | 0.299 ± 0.011 | 0.358 ± 0.036 | 0.247 ± 0.031 (ns) | 0.239 ± 0.028 | 0.347 ± 0.014a |
| BV/TV (%) | 21.62 ± 0.44 | 21.17 ± 0.54 | 24.93 ± 0.3.11 | 15.36 ± 0.99c | 16.45 ± 0.61 | 22.17 ± 0.81a |
| Tb.N (mm−1) | 4.22 ± 0.12 | 4.48 ± 0.20 | 4.57 ± 0.39 | 3.08 ± 0.32c | 3.16 ± 0.21 | 4.13 ± 0.12a |
| Tb.Pf (mm−1) | 7.08 ± 0.49 | 6.96 ± 1.03 | 6.82 ± 0.83 | 11.10 ± 1.01d | 10.51 ± 1.02 | 6.22 ± 0.37b |
| SMI | 1.133 ± 0.045 | 1.135 ± 0.044 | 0.985 ± 0.056 | 1.417 ± 0.057c | 1.361 ± 0.079 | 1.154 ± 0.058b |
| Conn.Dn (1/mm3) | 186.8 ± 15.46 | 205.2 ± 18.22 | 219.6 ± 19.28 | 105.9 ± 18.33d | 97.4 ± 12.01 | 161.7 ± 14.0a |
| Strength (mJ) | 213.7 ± 15.33 | 215.5 ± 11.60 | 261.7 ± 17.68 | 144.4 ± 15.04c | 160.6 ± 17.66 | 208.7 ± 7.38a |
| Stiffness (N/mm) | 696.6 ± 27.05 | 676.2 ± 28.98 | 788.9 ± 86.50 | 443.0 ± 30.54d | 476.8 ± 33.26 | 620.7 ± 35.48a |
miR-672-5p treatment restores bone loss and biomechanical properties in the vertebral bone of OVx mice at the end of 6 weeks of treatment. Table 1 shows volumetric bone-mineral density (vBMD), structural micro-CT parameters for trabecular bone, including bone volume to tissue volume (BV/TV), trabecular number (Tb.N), trabecular pattern factor (Tb.Pf), structure model index (SMI) and connection density (Conn.Dn), and bone biomechanical parameters like strength and stiffness (units are shown). Data are mean ± SE. ns, statistically non-significant; n = 6 mice per group. See also Figures 2 and S1.
ap < 0.05 and bp < 0.01 compared with the OVx-PBS-treated group or cp < 0.05 and dp < 0.01compared with the Sham-PBS-treated group.