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. 2019 Mar;368(3):423–434. doi: 10.1124/jpet.118.251876

Fig. 3.

Fig. 3.

Effect of PRCL diet on liver and kidney. Tissues were isolated from 2.9-month-old LCAT-KO × SREBP1a mice fed a regular chow (n = 3 for livers and 4 for kidney) or PRCL diet (n = 6 for liver and 5 for kidney) for 9 days. The PRCL diet markedly increased liver concentration of cholesterol (A) and triglycerides (B) (1.65 ± 0.4-fold and 2.8 ± 0.9-fold, respectively). Changes in protein concentration in liver (C) were statistically nonsignificant. Liver weight (D) was increased 2.6 ± 0.7-fold on the PRCL diet. Kidney weight (E) did not significantly increase after 9 days on the PRCL diet. (F) Cholesterol concentration in kidney did not significantly increase on the PRCL diet. (G) Expression of cholesterol metabolism genes Dhcr24, Hmgcr, and Ldlr in the kidney did not change on PRCL diet. The ratio of mRNA levels on the PRCL diet (n = 6) to levels on the chow diet (n = 4) are shown. *P < 0.05; **P < 0.01; ****P < 0.00001.