Fig. 4.

PRCL diet increases lipid accumulation and promotes the appearance of LpX in the liver of LCAT-KO × SREBP1a mice. (A) Liver from mice maintained on a normal chow diet. Note the accumulation of numerous lipid droplets in hepatocytes. (B–D) Livers from mice after 9 days on the PRCL diet. (B) Note the massive increase in hepatocyte lipid droplet accumulation. LpX particles were observed in liver sinusoids (C) containing RBCs and in bile canaliculi (D) with characteristic microvilli. LpX particles are clearly seen to be multilamellar in the insets (enlarged red boxes) in (C and D). RBC, red blood cell; MVs, microvilli. Scale bars (A and B) 4 µm; (C) 500 nm; (D) 1 µm; inset (C); 200 nm; inset (D); 500 nm.